Journal: Biological Trace Element Research

Article Title: The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms

doi: 10.1007/s12011-023-04057-5

Figure Lengend Snippet: Primers used in mouse RT-PCR experiments

Article Snippet: A 50 μL containing 5 × 10 4 cells as the unstained cell group was separated into a separate microcentrifuge tube and the remaining cells were stained with 1 μL of FITC mouse anti-human ZIP14 (MyBioSource, MBS151580) antibody and isotype antibodies respectively to the cells in 100 μL of 5% BSA and 0.05% sodium azide in 1X PBS.

Techniques:

Journal: Biological Trace Element Research

Article Title: The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms

doi: 10.1007/s12011-023-04057-5

Figure Lengend Snippet: Effects of ZnCl 2 on the accumulation of MTF-1, histone deacetylase 4 to the promoters of ZIP14 promoter. The Sham group received mineral oil only, CCl 4 and CCl 4 + ZnCl 2 group were injected with 10% CCl 4 in mineral oil for 8 weeks. 10 µM ZnCl 2 was only injected to CCl 4 + ZnCl 2 group for two weeks after CCl 4 treatment was completed. The enrichment of A MTF-1, B HDAC4 on the ZIP14 gene was analyzed by ChIP analysis. Genomic DNA extracted from hepatocytes of A control mice and B mice with CCl 4 -induced hepatic fibrosis were immunoprecipitated with anti-MTF-1 and anti-HDAC4. The change in the accumulation of these proteins on the ZIP14 gene was quantified by qPCR. The data were expressed as a percent of input. An asterisk (*) indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001. All data are represented as the mean ± SD ( n = 4)

Article Snippet: A 50 μL containing 5 × 10 4 cells as the unstained cell group was separated into a separate microcentrifuge tube and the remaining cells were stained with 1 μL of FITC mouse anti-human ZIP14 (MyBioSource, MBS151580) antibody and isotype antibodies respectively to the cells in 100 μL of 5% BSA and 0.05% sodium azide in 1X PBS.

Techniques: Histone Deacetylase Assay, Injection, Control, Immunoprecipitation

Journal: Biological Trace Element Research

Article Title: The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms

doi: 10.1007/s12011-023-04057-5

Figure Lengend Snippet: Changes in mRNA and protein expression of the zinc transporter ZIP14 upon ZnCl 2 treatment in mice with CCl 4 -induced hepatic fibrosis. The Sham group received mineral oil only, CCl 4 and CCl 4 + ZnCl 2 group were injected with 10% CCl 4 in mineral oil for 8 weeks. A 10 µM ZnCl 2 was only injected into the CCl 4 + ZnCl 2 group for two weeks after CCl 4 treatment was completed. A The mRNA expression levels of ZIP14 in were measured by qRT-PCR in hepatocytes. B The protein expression of ZIP14 was measured with flow cytometry and C supported with the immunofluorescence staining with ZIP14 antibody. Hepatocytes were isolated from control (sham) and fibrotic (CCl 4 ) mice were cultured. Transcript levels were normalized to GAPDH. * Indicates p < 0.05, ** indicates p < 0.01. All data are represented as the mean ± SD ( n = 4)

Article Snippet: A 50 μL containing 5 × 10 4 cells as the unstained cell group was separated into a separate microcentrifuge tube and the remaining cells were stained with 1 μL of FITC mouse anti-human ZIP14 (MyBioSource, MBS151580) antibody and isotype antibodies respectively to the cells in 100 μL of 5% BSA and 0.05% sodium azide in 1X PBS.

Techniques: Expressing, Injection, Quantitative RT-PCR, Flow Cytometry, Immunofluorescence, Staining, Isolation, Control, Cell Culture